Patent · US Active

T7 RNA polymerase variants with enhanced thermostability

US9193959B2 · kind B2 · utility

10Cited by

3References

11Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Harald Sobek · Penzberg, DE
Johann-Peter Thalhofer · Weilheim, DE
Rainer Mueller · Penzberg, DE
Manfred Schmidt · Krefeld, DE
Michael Greif · Penzberg, DE
Armin Ruf · Glückstadt, DE
Christian Rudolph · Penzberg, DE

Key dates

Filing date	Mar 23, 2011
Grant date	Nov 24, 2015
Priority date	—
Expiry date	Dec 9, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y207/07006
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.