Process for chromosomal engineering using a novel dna repair system
US9193974B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 10, 2006 |
| Grant date | Nov 24, 2015 |
| Priority date | — |
| Expiry date | Oct 19, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/74
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to chromosomal engineering via DNA repair process. The process of the invention comprises the steps of: 1) submitting at least one source of biological activity, e.g. Deinococcus radiodurans, to radiation, desiccation and/or chemical treatment liable to damage the DNA, so as to substantially shatter its chromosomes into short fragments; 2) annealing complementary single strand tails extended by the synthesis templated on partially overlapping DNA fragments of said shattered chromosomes; 4) converting the resulting long linear DNA intermediates into intact circular chromosomes, by means of a RecA dependent homologous recombination; whereas at least one foreign source of genetic material, e.g. DNA, can be introduced during steps 2 and/or 3; and 4) optionally separating and collecting the recombined chromosomes thus obtained.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.