Anti-cocaine compositions and treatment

US9200265B2 · kind B2 · utility

Assignees

• The Trustees of Columbia University in the City of New York · US
• THE REGENTS OF THE UNIVERSITY OF MICHIGAN · US
• University of Kentucky Research Foundation · US

Inventors

• Donald W. Landry · New York, US
• Chang-Guo Zhan · Nanhu, CN
• James H. Woods · Ann Arbor, US
• Roger K. Sunahara · Ann Arbor, US
• Diwahar L. Narasimhan · Ann Arbor, US
• Joanne MacDonald · Plymouth, US
• Victor C. Yang · Ann Arbor, US
• Mei-Chuan Holden Ko · Ann Arbor, US
• Shi-Xian Deng · White Plains, US
• John J. Tesmer · Ann Arbor, US
• Tien-Yi Lee · Ann Arbor, US
• Young Min Kwon · West Fork, US
• Daquan Gao · Louisville, US

Key dates

• Filing date: Nov 14, 2012
• Grant date: Dec 1, 2015
• Expiry date: May 6, 2033

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Embodiments of the invention disclosed herein generally relate to anti-cocaine therapeutics. Specifically, some embodiments of the invention relate to highly efficient, thermostable, and long-lasting cocaine esterase (CocE) mutants that can protect against the toxic and reinforcing effects of cocaine in subjects. Provided herein are mutant CocE polypeptides displaying thermostable esterase activity. Also provided are methods of treating cocaine-induced conditions in a subject in need via administration of mutant CocE as well as methods for high-throughput screening of candidate esterase polypeptides.