Synthetic siRNA detection method
US9200314B2 · kind B2 · utility
0Cited by
0References
5Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 20, 2011 |
| Grant date | Dec 1, 2015 |
| Priority date | — |
| Expiry date | Oct 11, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1096
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for accurately and easily detecting a synthetic siRNA, for example, a siRNA in which the 3′ end is DNA, and a kit used for the method are provided. The present invention relates to a method for detecting a siRNA in which the 3′ end is DNA, comprising: (a) adding polydeoxyadenosine to the 3′ DNA end of at least one strand of the siRNA to be detected to produce a polydeoxyadenosine-added RNA; (b) annealing a polydeoxythymidine primer having a tag sequence at its 5′ side to the polydeoxyadenosine-added RNA and synthesizing DNA from the primer by a reverse transcription; and (c) detecting the DNA synthesized in (b).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.