Patent · US Active

Hybridization methods using natural base mismatches

US9200323B2 · kind B2 · utility

0Cited by

3References

18Claims

0Family size

Assignees

CAPITALBIO CORPORATION · CN
TSINGHUA UNIVERSITY · CN

Inventors

Huafang Gao · Beijing, CN
Ze Li · Beijing, CN
Dong Wang · Hefei, CN
Yanhua Liu · Shanghai, CN
Xiang-Qian Liu · Collegeville, US
Yangzhou Jiang · Beijing, CN
Li Li · Beijing, CN
Chuanzan Zhao · Beijing, CN
Gengxin Lan · Beijing, CN
Tao Guo · Beijing, CN
Bin Cai · Beijing, CN
Jing Cheng · Beijing, CN

Key dates

Filing date	Feb 6, 2007
Grant date	Dec 1, 2015
Priority date	—
Expiry date	Jan 9, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/156
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides an improved nucleic acid hybridization process employing a modified oligonucleotide probe comprising naturally occurring nucleotide bases. At least one nucleotide in the modified oligonucleotide is artificially mismatched relative to the control nucleic acid in addition to any mismatches arising from a variant nucleic acid target containing a sequence variation. The artificial mismatch and the sequence variation positions are separated from one another on the oligonucleotide by six to nine nucleotide positions.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.