Enhanced citric acid production in aspergillus with inactivated asparagine-linked glycosylation protein 3 (ALG3), and/or increased laeA expression
US9206450B2 · kind B2 · utility
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Key dates
| Filing date | May 4, 2015 |
| Grant date | Dec 8, 2015 |
| Priority date | — |
| Expiry date | May 4, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y204/01258
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are fungi, such as Aspergillus niger, having a dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase (Alg3) gene genetic inactivation, increased expression of a loss of aflR expression A (Lae), or both. In some examples, such mutants have several phenotypes, including an increased production of citric acid relative to the parental strain. Methods of using the disclosed fungi to make citric acid are also provided, as are compositions and kits including the disclosed fungi.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.