Patent · US Active

Method for purifying active GLA-domain coagulation proteins

US9212365B2 · kind B2 · utility

1Cited by

1References

14Claims

0Family size

Assignee

Laboratoire Francais du Fractionnement et des Biotechnologies · FR

Inventors

Gerard Perret · Tampa, US
Nicolas Bihoreau · Orsay, FR
Laurent Siret · Épinay-sur-Orge, FR

Key dates

Filing date	Jul 30, 2010
Grant date	Dec 15, 2015
Priority date	—
Expiry date	Oct 13, 2030

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y304/21022
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A a method for purifying biologically active GLA-domain coagulation proteins, includes the following steps: a) bringing a sample that contains one or more GLA-domain coagulation proteins and may contain biologically inactive molecules of GLA-domain protein(s), into contact with an affinity substrate on which nucleic aptamers that bind specifically to at least one biologically active GLA-domain coagulation protein are immobilized, in order to form complexes between (i) the nucleic aptamers and (ii) the GLA-domain coagulation protein(s), b) releasing the GLA-domain coagulation protein(s) from the complexes formed in step a), and c) recovering the biologically active GLA-domain coagulation protein(s) in a purified form.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.