Biological detection system and method
US9212383B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Apr 4, 2008 |
| Grant date | Dec 15, 2015 |
| Priority date | — |
| Expiry date | Nov 8, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/32
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present disclosure, according to some embodiments, relates to phage-based biological detection systems, compositions, and methods. In some embodiments, it relates to a detection system and method using phage binding and bacterial infection to detect the presence of a target molecule (e.g., a toxin). One detection system may include a genetically engineered phage that expresses a surface molecule able to bind a target molecule and/or target microorganism; a bacterium susceptible to infection by the phage; and a detection component able to determine whether the bacterium has been infected by the phage. Infection of a bacterium by a phage may be indicative of phage binding to the target molecule and/or target microorganism. One method may include placing a sample suspected of containing the target molecule and/or target microorganism with a binder; adding a genetically engineered phage having reporter genetic material and able to bind the target molecule and/or target microorganism; washing away unbound phage; releasing phage bound to the target molecule and/or target microorganism; infecting a bacterium with the released phage; and detecting the presence of any reporter genetic m…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.