Assembly of high fidelity polynucleotides
US9217144B2 · kind B2 · utility
64Cited by
155References
25Claims
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Key dates
| Filing date | Jan 6, 2011 |
| Grant date | Dec 22, 2015 |
| Priority date | — |
| Expiry date | Jan 6, 2031 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00722
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.