D-psicose 3-epimerase mutant with improved thermal stability, and continuous production of D-psicose using same
US9217166B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 21, 2012 |
| Grant date | Dec 22, 2015 |
| Priority date | — |
| Expiry date | Aug 21, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y501/03
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a D-psicose 3-epimerase variant with improved thermostability by substituting an amino acid at a specific position of an amino acid sequence of a wild type D-psicose 3-epimerase. Further, the present invention provides a recombinant expression vector including a gene of the D-psicose 3-epimerase variant, and a recombinant strain transformed with the recombinant expression vector. Furthermore, the present invention provides an immobilized reactor prepared using the D-psicose 3-epimerase variant or the recombinant strain, and a method of continuously producing D-psicose using the immobilized reactor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.