Oligonucleotide primer with omega structure for detecting short-chain RNAs and use thereof
US9217180B2 · kind B2 · utility
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Key dates
| Filing date | Jan 16, 2013 |
| Grant date | Dec 22, 2015 |
| Priority date | — |
| Expiry date | Jan 16, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are an oligonucleotide primer with an omega structure for detecting short-chain RNAs and the use thereof. The primer from the 5′ end to the 3′ end sequentially is: a PCR primer target region of 20-30 bases, a variable coding region of 0-50 bases, an omega stem-loop, a probe spacer of at least one base and a probe region of 4-11 bases. The length of the stem of the omega stem-loop is 4-12 paired bases, and the length of the loop of the omega stem-loop is 3-20 unpaired bases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.