Mesenchymal stem cells (MSC) expansion methods and materials
US9220810B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 12, 2011 |
| Grant date | Dec 29, 2015 |
| Priority date | — |
| Expiry date | Oct 8, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2539/10
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
The subject invention concerns materials and methods for growing and expanding MSC while maintaining their undifferentiated phenotype, self-renewal ability, and/or multi-lineage potential. In one embodiment, a method of the invention comprises i) seeding freshly isolated MSC on a planar surface or a 3-D scaffold and growing the cells under physiological or low O2 tension for a period of time sufficient to support formation of 3-D ECM network; ii) decellularizing the planar surface or 3-D scaffold; and iii) reseeding the decellularized planar surface or 3-D scaffold with MSCs, whereby the reseeded MSCs can be grown on the scaffold and maintain an undifferentiated phenotype. In one embodiment, the 3-D scaffold comprises or is composed of PET. In one embodiment, the MSC are human MSC (hMSC).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.