Single use centrifuge system for highly concentrated and/or turbid feeds
US9222067B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 21, 2012 |
| Grant date | Dec 29, 2015 |
| Priority date | — |
| Expiry date | Mar 10, 2034 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB04B2005/0464
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method and apparatus for cell harvest of production scale quantities of cell cultures using single use components comprising a flexible membrane mounted on a rigid frame and is supported within a multiple use rigid centrifuge bowl, such single use components including a core with an increased diameter and an internal truncated cone shape in order to permit the system to maintain a sufficiently high angular velocity to create a settling velocity suited to efficiently processing highly concentrated cell culture streams. Features which minimize feed turbidity, and others which permit the continuous or semi-continuous discharge of cell concentrate, increase the overall production rate over the rate which can be achieved using current intermittent processing methods for large cell culture volumes. Injection of a diluent during the cell concentrate removal process permits more complete removal of viscous cell concentrates.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.