Patent · US Active

Method of producing and purifying an active soluble sialyltransferase

US9222081B2 · kind B2 · utility

0Cited by

2References

20Claims

0Family size

Assignee

BIOGENERIX GMBH · DE

Inventors

Axel Angermann · Dreieich, DE
Christian Scheckermann · Ehrenkirchen, DE
Karsten Schmidt · San Diego, US

Key dates

Filing date	Aug 2, 2011
Grant date	Dec 29, 2015
Priority date	—
Expiry date	Aug 2, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y204/99003
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a method for the production and purification of a sialyltransferase polypeptide, in particular a N-Acetylgalactosamine (Gal NAc)-α-2,6-sialyltransferase I (ST6GalNAcI) polypeptide. The method comprises the steps of producing the sialyltransferase polypeptide in a Chinese Hamster Ovary (CHO) cell and purifying the polypeptide with a combination of chromatography steps. The method results in high yield of sialyltransferase polypeptide which is highly pure and active. The obtained sialyltransferase, especially ST6GalNAcI, can be employed for the glycosylation of therapeutic proteins such as G-CSF.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.