Confocal photoacoustic microscopy with optical lateral resolution
US9226666B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 1, 2013 |
| Grant date | Jan 5, 2016 |
| Priority date | — |
| Expiry date | May 1, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2291/02872
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining a ratio of hemoglobin absorption within a biological tissue using a handheld device is provided. The method includes focusing at least one light pulse into a predetermined area inside an object using at least one excitation wavelength, wherein a fluorescence signal is based on at least a local excitation optical fluence and properties of fluorophores in the predetermined area. The method further includes receiving a photoacoustic signal emitted by the object in response to the at least one light pulse, the photoacoustic signal being a product of the local excitation optical fluence, an optical absorption coefficient of hemoglobin, and an acoustic detection sensitivity. The method further includes determining the ratio of hemoglobin absorption based on a hemoglobin absorption coefficient that is based at least partially on the fluorescence signal and the photoacoustic signal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.