Method for controlling the growth of cell culture
US9228167B2 · kind B2 · utility
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19Claims
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Key dates
| Filing date | Nov 29, 2007 |
| Grant date | Jan 5, 2016 |
| Priority date | — |
| Expiry date | Dec 7, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N1/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
In a method for controlling the growth of microbes grown in a medium to high cell-densities by the fed-batch technology, a two-phase system, having a liquid phase (cultivation medium) and a solid or gel phase, the solid or gel phase provides a source of substrate-delivering polymer which is turned by an enzyme in a controlled way into a growth-limiting substrate or pH adjusting agent. Also described is a method for restricting the synthesis of growth-limiting metabolites and preventing oxygen-depletion in microbial cell cultures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.