Plant transformation with in vivo assembly of a sequence of interest using a site-specific recombinase

US9228192B2 · kind B2 · utility

Assignee

• Bayer CropScience N.V. · BE

Inventors

• Anatoly Giritch · Halle (Saale), DE
• Serik Eliby · Bodenwerder, DE
• Sylvestre Marillonnet · Bodenwerder, DE
• Victor Klimyuk · Leipzig, DE
• Yuri Gleba · Berlin, DE

Key dates

• Filing date: Jan 30, 2004
• Grant date: Jan 5, 2016
• Expiry date: Feb 12, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8205
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process of producing transgenic plants or plant cells stably transformed on a chromosome with a DNA sequence of interest capable of expressing a function of interest, said process comprising (a) providing plant cells or plants with at least two different vectors that are adapted to recombine with each other between site-specific recombination sites compatible with a site-specific recombinase that is also provided in order to produce a non-replicating recombination product containing said DNA sequence of interest, (ii) said at least two different vectors are adapted for integrating said DNA sequence of interest into said chromosome, (iii) said DNA sequence of interest contains sequence portions from at least two of said at least two different vectors, said sequence portions being necessary for expressing said function of interest from said DNA sequence of interest; and (b) selecting plants or plant cells expressing said function of interest.