Enhanced gene expression
US9234195B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 25, 2011 |
| Grant date | Jan 12, 2016 |
| Priority date | — |
| Expiry date | Nov 25, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/907
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure is directed to a novel, unexpected approach of expressing exogenous gene(s) at increased levels by predictably, optionally irreversibly, incorporating the gene(s) into a region of increased gene expression (RIDGE) on a chromosome of a host cell. This approach is accomplished by identification of RIDGE(s) and further by integration of integrase-specific sites (e.g., attP or attB) in the presence of or mediated by integrase. The approach renders a high level of gene expression; predictability of the location of the exogenous genes, elimination of genetic instability or unwanted phenotype; and reduction of time and cost in optimizing protein production in host cells, which will be every useful in the production of therapeutic, prophylactic, and diagnostic proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.