Motif-specific and context-independent antibodies that specifically bind to a cleaved caspase motif

US9249231B2 · kind B2 · utility

Assignee

• CELL SIGNALING TECHNOLOGY, INC. · US

Inventors

• Michael Comb · Attleboro, US
• Ailan Guo · Lexington, US
• John Edward Rush, II · Beverly, US
• Jun-Ming Cai · Hamilton, US
• Jing Li · Lexington, US
• Jing Zhou · Sichuan, CN

Key dates

• Filing date: Nov 4, 2011
• Grant date: Feb 2, 2016
• Expiry date: Nov 4, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/34
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

There is provided a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) at least one sumoylated lysine residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said sumoylated lysine residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs. Also provided is a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) a C-terminal aspartic acid residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said C-terminal aspartic acid residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs.