Resonance energy transfer assay with cleavage sequence and spacer
US9249451B2 · kind B2 · utility
3Cited by
12References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 25, 2015 |
| Grant date | Feb 2, 2016 |
| Priority date | — |
| Expiry date | Feb 25, 2035 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/952
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A molecular construct comprises a donor label, an acceptor label, a linker peptide disposed between the donor and the acceptor, the linker having a cleavage site sequence, and a flexible peptide spacer between at least one of (a) the donor and the cleavage site sequence and (b) the acceptor and the cleavage site sequence. The cleavage site sequence is a protease cleavage site, and the flexible spacer peptide is positioned and dimensioned to facilitate an electronic hop between the donor and acceptor label.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.