Method and apparatus for automatic focusing of substrates in flourescence microscopy
US9250434B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 23, 2011 |
| Grant date | Feb 2, 2016 |
| Priority date | — |
| Expiry date | May 23, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/6458
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus for automated examination of biological material (6) includes a microscope (8) with a cross table (9) with the biological material (6) being arranged on the cross table (9) between an object slide (1) or an analysis plate and a cover (4); a light source (15, 16); an evaluation unit; a device for automatic focusing (11); an image recording unit (17) for recording an image of the biological material (6) enlarged via of an object lens (10) of the microscope (8) and for transferring the image to the evaluation unit; and a biochip (2). Labeling (7) is provided on a surface of the biochip (2). Focusing is executable by targeted movement of the cross table (9) based on detection of the labeling (7), and a target interval is determinable by determination of a location and/or a position of the labeling, within which focusing of the biological material takes place.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.