Absolute quantitation of proteins and protein modifications by mass spectrometry with multiplexed internal standards

US9252003B2 · kind B2 · utility

Assignee

• Pierce Biotechnology, Inc. · US

Inventors

• Greg Hermanson · Loves Park, US
• John Charles Rogers · Rockton, US
• Joel Louette · Ulm, DE
• Ryan Daniel Bomgarden · Winnebago, US
• Bhavinkumar Patel · Malmö, SE

Key dates

• Filing date: Jun 6, 2014
• Grant date: Feb 2, 2016
• Expiry date: Jul 30, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2458/15
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte.