Variant, recombinant beta-glucocerebrosidase proteins with increased stability and increased retained catalytic activity
US9254313B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 2, 2015 |
| Grant date | Feb 9, 2016 |
| Priority date | — |
| Expiry date | Feb 2, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01045
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Described herein are variant, recombinant β-glucocerebrosidase proteins characterized as having increased stability relative to recombinant wild-type β-glucocerebrosidase. Also provided herein are variant, recombinant β-glucocerebrosidase proteins characterized as retaining more catalytic activity relative to recombinant wild-type β-glucocerebrosidase. Further described herein are variant, recombinant β-glucocerebrosidase proteins that can have amino acid variations at one or more of the following positions: 316, 317, 321 and 145. Methods of making the variant, recombinant β-glucocerebrosidase proteins are also described as well as methods of treating patients having lysosomal storage diseases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.