Patent · US Active

Multi plasmid system for the production of influenza virus

US9255253B2 · kind B2 · utility

0Cited by
39References
21Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 27, 2013
Grant dateFeb 9, 2016
Priority date—
Expiry dateFeb 27, 2033

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2840/20
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.