Rapid and accurate analysis of protein sialylation
US9260743B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 5, 2012 |
| Grant date | Feb 16, 2016 |
| Priority date | — |
| Expiry date | Jul 5, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/938
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention relates to methods and kits for the analysis of the sialylation of gluco-proteins. The samples of gluco-protein are incubated separately under three conditions: with beta-galactosidase, with beta-galactosidase+alpha-sialidase, and without an enzyme. After the enzyme treatment, high performance anion exchange chromatography with pulsed amperometric detection (HPAEC PAD) is used to make a quantitative determination of the total galactose in the sample, the non sialylated galactose and the exogenous galactose in the medium. The determination of said values makes it possible to deduce the percentage of sialylation of the gluco-protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.