Real time PCR assay for detection of bacterial respiratory pathogens

US9260762B2 · kind B2 · utility

Assignee

• The United States of America as represented by the Secretary of the Department of Health and Human Services, Centers for Disease Control and Prevention · US

Inventors

• Jonas M. Winchell · Lilburn, US
• Agnes Warner · Lilburn, US
• Kathleen Thurman · Tifton, US

Key dates

• Filing date: Apr 15, 2011
• Grant date: Feb 16, 2016
• Expiry date: May 2, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for detecting Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp. are disclosed. A sample suspected of containing a nucleic acid of one or more of M. pneumoniae, C. pneumoniae, and Legionella spp. is screened for the presence or absence of that nucleic acid. Determining whether the M. pneumoniae, C. pneumoniae, or Legionella spp. nucleic acid is present in the sample can be accomplished by detecting hybridization between a M. pneumoniae probe (such as a CARDS toxin probe), a C. pneumoniae probe (such as a ArgR probe), or a Legionella spp. probe (such as a SsrA probe) and a nucleic acid in a sample. Also disclosed are probes and primers for the detection of M. pneumoniae, C. pneumoniae, and Legionella spp., and kits that contain the disclosed probes and/or primers.