Patent · US Active

Evolution of bond-forming enzymes

US9267127B2 · kind B2 · utility

67Cited by

1References

32Claims

0Family size

Assignee

President and Fellows of Harvard College · US

Inventors

David R. Liu · Lexington, US
Irwin Chen · Moreno Valley, US
Brent M. Dorr · Collegeville, US

Key dates

Filing date	Jun 20, 2013
Grant date	Feb 23, 2016
Priority date	—
Expiry date	Jun 20, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Y304/2207
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Strategies, systems, methods, reagents, and kits for the directed evolution of bond-forming enzymes are provided herein. Evolution products, for example, evolved sortases exhibiting enhanced reaction kinetics and/or altered substrate preferences are also provided herein, as are methods for using such evolved bond-forming enzymes. Kits comprising materials, reagents, and cells for carrying out the directed evolution methods described herein are also provided.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.