Ex vivo human multiple myeloma cancer niche and its use as a model for personalized treatment of multiple myeloma
US9267938B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 14, 2013 |
| Grant date | Feb 23, 2016 |
| Priority date | — |
| Expiry date | May 27, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/7028
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The described invention provides an ex vivo dynamic multiple myeloma (MM) cancer niche contained in a microfluidic device. The dynamic MM cancer niche includes (a) a three-dimensional tissue construct containing a dynamic ex vivo bone marrow (BM) niche, which contains a mineralized bone-like tissue containing viable osteoblasts self-organized into cohesive multiple cell layers and an extracellular matrix secreted by the viable adherent osteoblasts; and a microenvironment dynamically perfused by nutrients and dissolved gas molecules; and (b) human myeloma cells seeded from a biospecimen composition comprising mononuclear cells and the multiple myeloma cells. The human myeloma cells are in contact with osteoblasts of the BM niche, and the viability of the human myeloma cells is maintained by the MM cancer niche.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.