Method for controlling the biological activity of a protein in a vertebrate cell
US9273115B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 22, 2010 |
| Grant date | Mar 1, 2016 |
| Priority date | — |
| Expiry date | Jan 7, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/715
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor α, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site-specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.