Depletion of host cell components from live vector vaccines

US9273289B2 · kind B2 · utility

Assignee

• ProBioGen AG · DE

Inventors

• Ingo Jordan · Berlin, DE
• Holger Bernhardt · Berlin, DE
• Stefan Hartmann · Eggolsheim, DE

Key dates

• Filing date: Nov 23, 2011
• Grant date: Mar 1, 2016
• Expiry date: Dec 1, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2710/24151
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

It is desirable to produce live vaccines, which are highly attenuated and which do only contain minimal or no animal-derived components. The production of highly attenuated live viruses can be better achieved when using specifically designed cell lines as producer substrate as opposed to using less defined primary cells. However, live viruses, thus produced comprise undesirable components from the cell lines and cell culture. The present invention relates to methods of production and purification of live enveloped viruses, which are suitable for vaccination.