Meganuclease variants cleaving a DNA target sequence from a glutamine synthetase gene and uses thereof
US9273296B2 · kind B2 · utility
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7References
31Claims
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Key dates
| Filing date | Sep 8, 2008 |
| Grant date | Mar 1, 2016 |
| Priority date | — |
| Expiry date | Mar 13, 2031 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P43/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An I-CreI variant, wherein one of the two I-CreI monomers has at least two substitutions, one in each of the two functional subdomains of the LAGLIDADG core domain situated respectively from positions 28 to 40 and 44 to 77 of I-CreI, said variant being able to cleave a DNA target sequence from the glutamine synthetase gene. Use of said variant and derived products for improving expression system for the production of recombinant protein.
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