Methods for cloning and manipulating genomes

US9273310B2 · kind B2 · utility

Assignee

• Synthetic Genomics, Inc. · US

Inventors

• Gwynedd A. Benders · Portland, US
• John I. Glass · San Diego, US
• Clyde A. Hutchison · Village of La Jolla, US
• Carole Lartigue · Gaithersburg, US
• Sanjay Vashee · Boyds, US
• Mikkel A. Algire · Jessup, US
• Hamilton O. Smith · San Diego, US
• Charles E. Merryman · Beverly Farms, US
• Vladimir N. Noskov · Rockville, US
• Ray-Yuan Chuang · Rockville, US
• Daniel G. Gibson · Carlsbad, US
• J. Craig Venter · Village of La Jolla, US

Key dates

• Filing date: Mar 5, 2010
• Grant date: Mar 1, 2016
• Expiry date: Mar 5, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/81
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions and methods are disclosed herein for cloning a donor genome in a heterologous host cell. In one embodiment, the donor genome can be further modified within a host cell. Modified or unmodified genomes can be further isolated from the host cell and transferred to a recipient cell. Methods disclosed herein can be used to alter donor genomes from intractable donor cells in more tractable host cells.