Recombinant host cells having an increase in buoyant density

US9273336B2 · kind B2 · utility

Assignee

• E.I. DU PONT DE NEMOURS AND COMPANY · US

Inventors

• Ritu Bhalla · Malkajgiri, IN
• Qi Chen · Hangzhou City, CN
• Qiong Cheng · Wilmington, US
• Neeraj Pandey · Barwala, IN
• Pierre E. Rouviere · Wilmington, US
• Kristin Ruebling-Jass · Wilmington, US
• Annapurna Sachan · Kanpur, IN

Key dates

• Filing date: Jun 21, 2011
• Grant date: Mar 1, 2016
• Expiry date: Mar 10, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/70
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods are provided to obtain recombinant microbial cells having at least one genetic modification that increase the buoyant density of a recombinant microbial cell or the buoyant density of inclusion bodies produced within a recombinant microbial cell. Exemplified are genetic modifications that increase the buoyant density of a recombinant microbial cell expressing heterologous peptides and polypeptides. Increasing expression of the genes ysaB, glyQ, glyS or a combination thereof within the recombinant microbial cell produces cells or inclusion bodies having higher buoyant density. A similar effect was achieved by decreasing or disrupting expression of the endogenous gltA gene. Increases in buoyant density render peptide production more efficient with respect to time and costs.