Signal sequences to improve protein expressions and secretion of recombinant enzymes and other proteins
US9279007B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Nov 22, 2011 |
| Grant date | Mar 8, 2016 |
| Priority date | — |
| Expiry date | Nov 22, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/036
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Polypeptide signal sequences of modified fragments of human immunoglobulin heavy chain binding protein (Bip) are disclosed. Also disclosed are fusion proteins comprising a modified fragment of human immunoglobulin heavy chain binding protein (Bip) operably linked to a heterologous polypeptide. Also disclosed are protein expression vectors comprising a promoter operably linked to a first DNA sequence encoding a signal sequence comprising a modified fragment of human immunoglobulin heavy chain binding protein (Bip) and a second DNA sequence encoding a heterologous polypeptide fused in frame to the first DNA sequence. Further disclosed are methods of producing a polypeptide comprising expressing a fusion protein comprising a polypeptide signal sequences of modified fragments of human immunoglobulin heavy chain binding protein (Bip) operably linked to a heterologous polypeptide and recovering the heterologous polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.