Chromatographic method for purifying FC-containing proteins

US9284347B2 · kind B2 · utility

Assignee

• Boehringer Ingelheim International GmbH · DE

Inventors

• Christian Eckermann · Biberach, DE
• Dorothee Ambrosius · Munich, DE
• Franz Nothelfer · Biberach an der Riß, DE
• Thomas Rathjen · Biberach an der Riß, DE

Key dates

• Filing date: Jan 21, 2011
• Grant date: Mar 15, 2016
• Expiry date: Jul 28, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/14
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention relates to methods of depleting impurities, in particular host cell proteins (HCP) and DNA from cell culture supernatants by means of protein A chromatography using a novel washing buffer.