Method for producing laccase by means of liquid fermentation of Lentinus edodes

US9284535B2 · kind B2 · utility

Assignee

• Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences · CN

Inventors

• Qiang Yao · Baton Rouge, US
• Zhiyuan Gong · Hangzhou City, CN
• Zhaohui Liu · 安丰镇, CN
• Jiandong Han · Jinan, CN
• Qi Wang · Chesterfield, US
• Xiao Liu · Sichuan, CN
• Luchang Wan · Jinan, CN
• Pengfei Ren · Jinan, CN
• Haixia Ren · Shanghai, CN
• Jin Li · Sammamish, US
• Peng-Jian Yang · Hsinchu, TW
• Tao Sun · Naperville, US

Key dates

• Filing date: Mar 22, 2013
• Grant date: Mar 15, 2016
• Expiry date: Jul 15, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N1/14
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for producing laccase by liquid fermentation of Lentinus edodes comprises the following steps of: (1) inoculating strains of Lentinus edodes into the PD liquid fermentation medium to perform activation culture and obtain a seed liquid; (2) inoculating the seed solution into a liquid fermentation medium to perform liquid fermentation culture, then adding an expansin solution to perform further culture, separating the supernatant to obtain a ferment solution; (3) extracting laccase from the ferment solution. A plant expansin and an optimized fermentation method are applied for producing laccase by liquid fermentation of Lentinus edodes, which increases the liquid fermentation output of laccase of Lentinus edodes remarkably, by more than 3 times in comparing with traditional fermentation production methods.