Methods and compositions for protein labeling using lipoic acid ligases

US9284541B2 · kind B2 · utility

Assignees

• Massachusetts Institute of Technology · US
• University of Washington through its Center for Commercialization · US

Inventors

• Alice Y. Ting · Brookline, US
• David A. Baker · Nes Ziona, IL
• Florian Richter · Mannheim, DE
• Lucas Nivon · Seattle, US
• Daniel Liu · Vancouver, CA

Key dates

• Filing date: Oct 6, 2011
• Grant date: Mar 15, 2016
• Expiry date: Oct 18, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/9015
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present disclosure provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs (e.g., lipoic acid analogs comprising a resorufin moiety) recognized by lipoic acid ligase and lipoic acid ligase mutants. Also provided herein is a method of imaging protein-protein interaction via a reaction mediated by lipoic acid ligase.