Measuring range extension of chromatographic rapid tests

US9285376B2 · kind B2 · utility

Assignee

• Roche Diagnostics Operations, Inc. · US

Inventors

• Jurgen Spinke · Lorsch, DE
• Marcel Thiele · Rheda-Wiedenbrück, DE
• Jurgen Schaffler · Weinheim, DE
• Andreas Nufer · Bad Dürkheim, DE

Key dates

• Filing date: Jul 14, 2014
• Grant date: Mar 15, 2016
• Expiry date: Jul 14, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/557
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for the quantitative determination of an analyte in a sample is provided comprising: (a) providing an analyte-specific substance which is able to undergo a reaction which generates a detectable signal when it is contacted with an analyte; (b) providing at least two calibration graphs which have been generated by reacting in each case the same analyte-specific substance with different amounts of in each case the same test analyte for in each case a predetermined reaction time; (c) contacting the analyte-specific substance with a sample which contains the analyte to be detected; (d) measuring the signal at a first predetermined reaction time for which a first calibration graph according to (b) is provided; (c) checking whether the signal measured according to (d) enables a quantitative determination of the analyte with a desired accuracy; (f) (i) quantitatively determining the analyte on the basis of the signal measured according to (d) if the desired accuracy is reached, or (ii) measuring the signal at a second predetermined reaction time for which a second calibration graph according to (b) is provided; (g) checking whether the signal measured according to (f(ii)) enables …