Rapid detection of replicating cells
US9290382B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 1, 2009 |
| Grant date | Mar 22, 2016 |
| Priority date | — |
| Expiry date | Nov 12, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/33
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.