Amplicon melting analysis with saturation dyes
US9290663B2 · kind B2 · utility
2Cited by
37References
16Claims
0Family size
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Key dates
| Filing date | Mar 8, 2012 |
| Grant date | Mar 22, 2016 |
| Priority date | — |
| Expiry date | Mar 8, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.