Simplified methods for generating endothelial cells from human pluripotent stem cells under defined conditions
US9290741B2 · kind B2 · utility
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Key dates
| Filing date | Mar 14, 2014 |
| Grant date | Mar 22, 2016 |
| Priority date | — |
| Expiry date | Mar 14, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2506/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for generating human endothelial cells from human pluripotent stem cells under defined conditions in the absence of VEGF are described. Wnt/β-catenin signaling is activated in human pluripotent stem cells for a defined period, e.g., by inhibition of Gsk3, and then cultured without further exogenous activation of Wnt/β-catenin signaling to obtain a cell population containing human endothelial cells.
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