FRET measurement device and FRET measurement method
US9291563B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 22, 2013 |
| Grant date | Mar 22, 2016 |
| Priority date | — |
| Expiry date | Jun 9, 2033 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6441
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
FRET measurement uses a FRET probe that includes a probe element X containing a donor fluorescent substance and a probe element Y containing an acceptor fluorescent substance and enables FRET to occur when the probe element X and the probe element Y approach to each other or bind together. The modulation frequency of laser light with which the FRET probe is irradiated is adjusted to an optimum modulation frequency that maximizes a difference between the phase difference of donor fluorescence emitted from the donor fluorescent substance with respect to intensity modulation of the laser light at the time when FRET occurs and the phase difference of donor fluorescence emitted from the donor fluorescent substance with respect to intensity modulation of the laser light at the time when FRET does not occur.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.