Method for measuring protozoan oocyst and detecting reagent
US9297799B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 11, 2006 |
| Grant date | Mar 29, 2016 |
| Priority date | — |
| Expiry date | Nov 5, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/56905
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method for measuring oocyst of protozoa, such as Cryptosporidium, in an environment sample with high sensitivity at low cost within a short period of time; and a detecting reagent for use therein.Magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, binding factors for specific recognition of oocyst are added to an analyte containing a protozoan oocyst to form oocyst/binding factor/magnetic fine particle complexes by using a binding reaction to the oocyst, the formed complexes are recovered by a magnetic separation, and the protozoan oocysts contained in the complexes are assayed. Further, there is provided, for conducting the above method, a reagent for detecting protozoan oocysts comprising magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, antibodies against oocysts or binding factors for recognizing the antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.