Protein analysis using a polymethine marker dye

US9297807B2 · kind B2 · utility

Assignee

• AGILENT TECHNOLOGIES, INC. · US

Inventors

• Martin Kratzmeier · Waldbronn, DE
• Xiaodan Tian · Karlsruhe, DE
• Fritz Bek · Remchingen, DE

Key dates

• Filing date: Feb 10, 2006
• Grant date: Mar 29, 2016
• Expiry date: Jan 3, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/92
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A kit for optically detecting proteins, in particular lipoproteins, in a sample, the kit comprising: a chip for performing a separation of the proteins, in particular of the lipoproteins, wherein the chip comprises at least one well for receiving the sample, and a separation channel coupled to the at least one well and being adapted for separating different compounds and a marker dye which contains a polymethine of the general formula (I) wherein Z is a substituted derivative of benzooxazole, benzodiazole, 2,3,3-trimethylindolinine, 2,3,3-trimethyl-4,5-benzo-3H-indolinine, 3- and 4-picoline, lipidine, chinadine and 9-methylacridine derivatives with the general formula IIa or IIb or IIc and wherein X is selected from the group consisting of O, S, Si, N-alkyl and C(alkyl)2, n is 0, 1 , 2 or 3, R1 to R14 are independently selected from the group consisting of hydrogen, alkyl, alkoxy, cycloalkyl, linear or branched alkenyl, cycloalkenyl, aryl, heteroaryl, heterocycle, hydroxy, carboxy, amine, alkyl-substituted amine and cyclic amine and/or two or more fragments in ortho-position to each other, for example R10 and R11 or R4, R5 and R6, together form another cycloalkyl ring or ring syste…