Intein-modified enzymes, their production and industrial application
US9303250B2 · kind B2 · utility
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Key dates
| Filing date | Feb 26, 2013 |
| Grant date | Apr 5, 2016 |
| Priority date | — |
| Expiry date | Jul 18, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01008
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of predicting an intein insertion site in a protein that will lead to a switching phenotype is provided. The method includes identifying a plurality of C/T/S sites within the protein; selecting from the plurality of C/T/S/ sites those that are ranked 0.75 or higher by a support vector machine, within ten angstroms of the active site of the protein, and at or near a loop-β-sheet junction or a loop-α-helix junction. A method of controlling protein activity and hosts including proteins with controlled activity are also provided. Also, intein modified proteins and plants containing intein modified proteins are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.