Patent · US Active

All-in-one sample preparation device and method

US9304070B2 · kind B2 · utility

2Cited by
33References
16Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 27, 2012
Grant dateApr 5, 2016
Priority date
Expiry dateAug 3, 2034

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10T436/25375
  • WIPO fieldChemical engineering
  • WIPO sectorChemistry

Abstract

Sample preparation device that allows for a complete bind, wash, elute, buffer-exchange and concentration process to be carried out without sample transfer between multiple devices. The device includes a reservoir, a column for holding chromatography media, a holder region for holding a filtration device, and an outlet. The filtration device plugs into the holder region of the centrifugal device, and the assembly can be placed in an optional holder. The assembly, with or without the optional holder, can be placed in a conventional centrifuge tube for centrifugation. The entire bind, wash, elute, buffer exchange and concentration steps can be carried out with the apparatus without any pipette transfers (and the associated sample losses. The sample preparation device also can be used for binding and washing steps, in which case the filtration device is not needed, and for buffer exchange and concentration steps, in which case the media is not needed.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.