Methods and devices for analyte detection
US9304133B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 22, 2011 |
| Grant date | Apr 5, 2016 |
| Priority date | — |
| Expiry date | Feb 8, 2032 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0487
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods and apparatus are provided to resolve analytes within a fluid path using isoelectric focusing, gel electrophoresis, or other separation means. Materials within the fluid path that are compatible with these separation means are used to attach resolved analytes to the wall of the fluid path. Attachment results from a triggerable event such as photoactivation, thermal activation, or chemical activation. In accordance with a further aspect of the present invention, the material in the capillary may also be disrupted, by either the triggerable event or a subsequent event such as melting or photocleavage. Thus, an open lumen or porous structure may be created within the fluid path, allowing unbound analyte materials to be washed from the fluid path, and detection agents to be washed into the fluid path. The separation-compatible materials may be polymerizable monomers, gels, entangled polymers or other materials.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.