Preparation of an esterase

US9309503B2 · kind B2 · utility

Assignee

• DPx Holdings B.V. · NL

Inventors

• Martin Kietzmann · Graz, AT
• Helmut Schwab · Graz, AT
• Harald Pichler · Wolfsberg, AT
• Mirela Ivancic · Stari Grad, HR
• Oliver May · Frankfurt, DE
• Rudolf Gijsbertus Marie Luiten · Leiden, NL

Key dates

• Filing date: Jul 4, 2008
• Grant date: Apr 12, 2016
• Expiry date: Oct 28, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y301/01001
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a recombinant E. coli strain characterized in that the organisms contains a gene coding for a protein having esterase activity. Such E. coli is suitable for the preparation of a protein with esterase activity, whereby the expression takes place without coexpression of GroEL and/or GroES from a plasmid. The expression of glutathione reductase and/or thioredoxin reductase may be abolished by mutation. Furthermore, the ability of the organism to grow under oxygen-rich conditions has been restored by mutation. The expression of the gene of the protein with esterase activity is unaccompanied by the expression of an additional gene encoding a heat shock chaperone protein.