Methods, compositions, systems, apparatuses and kits for nucleic acid amplification

US9309566B2 · kind B2 · utility

Assignee

• Life Technologies Corporation · US

Inventors

• Bin Li · Columbus, US
• Kai Qin Lao · Pleasanton, US
• Jennifer O'Neil · Peabody, US
• Jennifer Kunkel · Burlington, US
• Kellie Haley · Peabody, US
• Rachel Kasinskas · Amesbury, US
• Zhaochun Ma · Sunnyvale, US
• Pius Brzoska · Woodside, US

Key dates

• Filing date: Mar 14, 2013
• Grant date: Apr 12, 2016
• Expiry date: Mar 14, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In some embodiments, the present teachings provide methods for paired end sequencing. In some embodiment, a polynucleotide template to be subjected to paired end sequencing comprises at least one cross linking moiety and at least one scissile moiety. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cleavage step, and (c) a reverse sequencing step. In some embodiments, a paired end sequencing reaction comprises (a) a forward sequencing step, (b) a cross-linking step, (c) a cleavage step, and (d) a reverse sequencing step.