Method and device for genetic map construction, method and device for haplotype analysis
US9309570B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 24, 2012 |
| Grant date | Apr 12, 2016 |
| Priority date | — |
| Expiry date | Aug 24, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG16B30/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are the method and device for genetic map construction and the method and device for haplotype determination of a single cell. Wherein the method for genetic map construction includes: whole genome sequencing for at least a single cell from a same species, aligning the sequencing data to reference sequences respectively to determine genotypes of SNP sites, determining male parent a/female parent b typing results of SNP genotypes of a single cell based on the genotypes of SNP sites, dividing the chromosome of the species into linkage regions based on the male parent a/female parent b typing results of SNP genotypes, determining the variation ratio of a/b between two linkage regions to obtain recombination rate between every two continuous linkage regions, determining recombination map of a single cell according to the recombination rate, wherein the boundary site of a and b is the recombination site, determining the recombination rate of each recombination rate based on the recombination map to construct a genetic map of the species.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.